Amyloidogenicity and Cytotoxicity of Recombinant Mature Human Islet Amyloid Polypeptide (rhIAPP)
نویسندگان
چکیده
منابع مشابه
Selection for nonamyloidogenic mutants of islet amyloid polypeptide (IAPP) identifies an extended region for amyloidogenicity.
The aggregation of the 37-residue protein, islet amyloid polypeptide (IAPP), as either insoluble amyloid or as small oligomers, appears to play a direct role in the death of pancreatic beta-islet cells in type II diabetes. While IAPP has been known to be the primary component of type II diabetes amyloid, the molecular interactions responsible for this aggregation have not been identified. To id...
متن کاملProtective role of human insulin against the cytotoxicity associated with human mutant S20G islet amyloid polypeptide
AIMS/INTRODUCTION Islet amyloid polypeptide (IAPP) is a main component of islet amyloid in type 2 diabetes and cosecreted from β-cell with insulin. Clinical evidence from the patients with S20G mutation of the IAPP gene, as well as experimental evidence that insulin could inhibit amyloid formation of IAPP, suggests that a gradual reduction of insulin could be related to the cytotoxicity associa...
متن کاملIslet amyloid polypeptide, islet amyloid, and diabetes mellitus.
Islet amyloid polypeptide (IAPP, or amylin) is one of the major secretory products of β-cells of the pancreatic islets of Langerhans. It is a regulatory peptide with putative function both locally in the islets, where it inhibits insulin and glucagon secretion, and at distant targets. It has binding sites in the brain, possibly contributing also to satiety regulation and inhibits gastric emptyi...
متن کاملElectrochemical Assay of Human Islet Amyloid Polypeptide and Its Aggregation
Square wave voltammetry is used in this work to detect human islet amyloid polypeptide (hIAPP) by using the oxidized signal of the tyrosine residue in hIAPP. A detection limit of 1×10-6 M for hIAPP has been obtained. A kinetic study of the aggregation process has been carried out according to the relationship between the anodic peak current in the square wave voltammograms of hIAPP and the incu...
متن کاملGenetic background determines the extent of islet amyloid formation in human islet amyloid polypeptide transgenic mice.
Genetic background is important in determining susceptibility to metabolic abnormalities such as insulin resistance and beta-cell dysfunction. Islet amyloid is associated with reduced beta-cell mass and function and develops in the majority of our C57BL/6J x DBA/2J (F(1)) male human islet amyloid polypeptide (hIAPP) transgenic mice after 1 yr of increased fat feeding. To determine the relative ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2004
ISSN: 0021-9258
DOI: 10.1074/jbc.m406108200